Conditional hepatocarcinogenesis in mice expressing SV 40 early sequences

We closely mimicked the in vivo setting in which sporadic hepatocarcinoma occurs by establishing a transgenic mouse model carrying regulatable SV40 early sequences under the control of the regulatory sequencesof the human antithrombinIII gene that confer hepatic expression. In this system, floxeddormant oncogenicsequences became functional after excision due to adenoviral expression of Cre recombinaseor the stable transgenic expression in liver of a tamoxifen-inducible Cre. Hepatic oncogeneexpression was switched on by both methods, leading to the development of hepatocellular carcinoma. This model could be useful for investigating the key steps of the preneoplastic process, to identify suitable targets for the testing of new therapies.