Large scale systematic proteomic quantification from non-metastatic to metastatic colorectal cancer
2015
A systematic proteomic quantification of formalin-fixed, paraffin-embedded (FFPE)
colorectal cancertissues from stage I to stage IIIC was performed in large scale. 1017 proteins were identified with 338 proteins in quantitative changes by label free method, while 341 proteins were quantified with significant expression changes among 6294 proteins by iTRAQ method. We found that proteins related to migration expression increased and those for binding and adherent decreased during the
colorectal cancerdevelopment according to the gene ontology (GO) annotation and
ingenuity
pathway analysis(IPA). The integrin alpha 5 (ITA5) in integrin family was focused, which was consistent with the metastasis related pathway. The expression level of ITA5 decreased in metastasis tissues and the result has been further verified by
Western blotting. Another two cell migration related proteins
vitronectin(VTN) and actin-related protein (ARP3) were also proved to be up-regulated by both mass spectrometry (MS) based quantification results and
Western blotting. Up to now, our result shows one of the largest dataset in
colorectal cancerproteomics research. Our strategy reveals a disease driven
omics-pattern for the metastasis
colorectal cancer.
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