Adipose-Derived Stromal Vascular Fraction Cell Effects on a Rodent Model of Thin Endometrium.
2015
Endometrial dysfunction affects approximately 1% of infertile women, and there is currently no standard therapy for improving fertility treatment outcomes in these patients. In our study, we utilized a rodent model of thin
endometriumto test whether intrauterine application of adipose-derived
stromal vascular fractioncells (SVF) could improve morphological and physiological markers of endometrial receptivity. Using
anhydrous ethanol, endometrial area and gland density were significantly reduced in our model of thin
endometrium. Application of SVF was associated with a 29% reduction in endometrial vascular endothelial growth factor (VEGF) expression and significant increases in
uterine arterysystolic/diastolic velocity ratios and
resistance indexvalues, suggesting reduced diastolic microvascular tone. However, no significant improvements in endometrial area or gland density were observed following SVF treatment. 3D confocal imaging demonstrated poor engraftment of SVF cells into recipient tissue, which likely contributed to the negative results of this study. We suspect modified treatment protocols utilizing adjuvant estrogen and/or tail vein cell delivery may improve SVF retention and therapeutic response in subsequent studies. SVF is an easily-obtainable cell product with regenerative capability that may have a future role in the treatment of infertile women with endometrial dysfunction.
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