Transcriptome analysis reveals class IX ethylene response factors show specific up-regulation in resistant but not susceptible Medicago truncatula lines following infection with Rhizoctonia solani
2018
The fungal pathogen
Rhizoctonia solaniAG8 causes substantial losses to cereal and
legumeproduction in Australia and the Pacific Northwest of the United States of America. Mutant analyses have revealed a critical role for ethylene mediated defence signalling for resistance to R. solani AG8 in the model
legume
Medicago truncatulawhich is, at least in part, mediated by ethylene dependent accumulation of
isoflavonoids. In this study we investigate the potential for members of the ethylene response transcription factor (ERF) family in mediating the
isoflavonoidand defence response. A strong and early
Rhizoctonia-responsive expression pattern was observed for many of the class IX ERFs in the moderately resistant wild type line A17, while the ethylene insensitive and highly susceptible mutant sickle (skl) showed a very limited regulation of this class. Conversely, the skl mutant demonstrated up-regulation of class II ERFs known to act as transcriptional repressors. Analysis of the presence of the GCC box promoter element, thought to be responsible for ERF binding and transcriptional activity, in genes differentially regulated in A17 suggests indirect or alternative mechanisms of ERF mediated gene regulation may be contributing to the large scale transcriptional adaptation of A17 following R. solani AG8 infection. Comparison of the expression profile with that following infection of A17 and skl with the symbiotic bacterium
Sinorhizobium medicaesuggests that
legumeshave adapted the ERF family to perform diverse roles to balance defence against pathogens and symbiosis with beneficial microorganisms in the same root tissue.
Keywords:
-
Correction
-
Source
-
Cite
-
Save
43
References
2
Citations
NaN
KQI