Whole-body scanning PCR; a highly sensitive method to study the biodistribution of mRNAs, noncoding RNAs and therapeutic oligonucleotides
2013
Efficient tissue-specific delivery is a crucial factor in the successful development of therapeutic
oligonucleotides. Screening for novel delivery methods with unique tissue-homing properties requires a rapid, sensitive, flexible and unbiased technique able to visualize the in vivo
biodistributionof these
oligonucleotides. Here, we present whole body scanning PCR, a platform that relies on the local extraction of tissues from a mouse whole body section followed by the conversion of target-specific qPCR signals into an image. This platform was designed to be compatible with a novel RT-qPCR assay for the detection of siRNAs and with an assay suitable for the detection of heavily chemically modified
oligonucleotides, which we termed
Chemical-LigationqPCR (CL-qPCR). In addition to this, the platform can also be used to investigate the global expression of endogenous mRNAs and
non-coding RNAs. Incorporation of other detection systems, such as aptamers, could even further expand the use of this technology.
Keywords:
-
Correction
-
Source
-
Cite
-
Save
39
References
12
Citations
NaN
KQI