MP32-04 IMMUNE CHECKPOINT B7X (B7-H4/B7S1/VTCN1) IS HIGHLY EXPRESSED IN SPONTANEOUS CANINE BLADDER CANCER: THE FIRST REPORT AND ITS IMPLICATIONS IN A PRECLINICAL MODEL

2019 
INTRODUCTION AND OBJECTIVES:Canine bladder cancer provides a novel model system for the study of invasive human urothelial carcinoma. The immunobiology of canine tumors is largely unknown. We report the first evaluation of immune checkpoint B7x, a major contributor to T-cell co-inhibition in human bladder cancers, in primary canine urothelial neoplasms.METHODS:With approval of the Purdue Animal Care and Use Committee, dogs with spontaneous bladder cancer were identified and tumor specimens were collected by cystoscopic biopsy from treatment-naive dogs. Normal bladder tissue was collected from dogs that were being euthanized for conditions other than bladder-related diseases. RNA-seq data from 29 canine invasive bladder tumors and 4 normal bladders were used to determine B7x expression. Gene ontology analysis was performed to explore the biological role of B7x. A set of 50 canine bladder tumor samples and 4 normal bladder tissues were evaluated for B7x protein by IHC and corresponding clinicopathologic dat...
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