Multicolor Labeling in Developmental Gene Regulatory Network Analysis
2014
The
sea urchinembryo is an important model system for developmental
gene regulatory network(GRN) analysis. This chapter describes the use of multicolor
fluorescentin
situ hybridization(FISH) as well as a combination of FISH and
immunohistochemistryin
sea urchinembryonic GRN studies. The methods presented here can be applied to a variety of experimental settings where accurate spatial resolution of multiple
gene productsis required for constructing a developmental GRN.
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