Improvement of terrestrial groundwater sampling method affects microbial community analysis
2019
AbstractGroundwater sampling is a critical step in subsurface
microbial ecology. Here, we compared two different sampling methods: commonly used disposable bailers (unimproved sampler) and an improved sampler, the latter of which was devised to minimize exposure to the aerobic atmosphere. Microbial community analysis using the 16S rRNA and
methyl coenzyme-M reductase(mcrA) genes in the lignite seam groundwater revealed that the archaeal communities in samples obtained by the improved sampler were dominated by
hydrogenotrophic
methanogen
Methanobacterium. These results suggested that the improved sampler would be more favorable for obtaining
methanogenicarchaeal community than the unimproved one, and that the sampling method affected the microbial community analysis in the investigated subterranean lignite seams.
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