Induction of puberty by autograft of cryopreserved ovarian tissue.

Summary of results of oestradiol, FSH, LH, inhibin B, and AMH from the day of transplantation to day 4 (D4) of the second spontaneous menstrual period future pubic hair, between the abdominal skin and the abdominal muscle. An abdominal pocket was opened and three thawed ovarian fragments were deposited inside. Hormonal investigations (fi gure) on the fi rst day of the ovarian transplantation showed FSH 89 IU/L, LH 36 IU/L, oestradiol less than 37 pmol/L, and AMH less than 0∙18 pmol/L. 2 months after the graft, breast growth started bilaterally, and she had reached Tanner stage S2 4 months after the graft. At this time, pubic and axillary hair appeared. H er fi rst menstruation was 8 months af ter transplantation, lasting 4 days. Breast development reached Tanner stage S3. At 3 years and 3 months after ovarian autotransplantation, our patient was 172 cm tall and weighed 52 kg. Her menstruation was regular for 2 years after the graft and irregular thereafter. Her breasts were completely developed with normal shape.Cryopreservation of ovarian tissue before potentially sterilising treatment can preserve ovarian function. 13 children have been born worldwide after auto-transplantation of ovarian fragments.