Proteomic profiling of human cancer pseudopodia for the identification of anti-metastatic drug candidates

Cancermetastasis causes approximately 90% of all cancer-related death and independent of the advancement of cancertherapy, a majority of late stage patients suffers from metastatic cancer. Metastasis implies cancer cell migrationand invasion throughout the body. Migration requires the formation of pseudopodiain the direction of movement, but a detailed understanding of this process and accordingly strategies of prevention remain elusive. Here, we use quantitative proteomicprofiling of human cancer pseudopodiato examine this mechanisms essential to metastasis formation, and identify potential candidates for pharmacological interference with the process. We demonstrate that Prohibitins(PHBs) are significantly enriched in the pseudopodiafraction derived from cancercells, and knockdown of PHBs, as well as their chemical inhibition through Rocaglamide(Roc-A), efficiently reduces cancer cell migration.