Appearance of the oocyte activation ability of mouse round spermatids cultured in Vitro
2008
This study was undertaken to determine the expression time of fertilization and
oocytes activationabilities of
spermatidsin the mouse. When
elongatingor
elongated
spermatidsisolated from fresh testes of adult males (B6D2F1) were injected into mature mouse
oocytes, both
spermatidscould activate the mature
oocytesand occur fertilization. On the one hand, the
round
spermatidscould not activate mature
oocytes, when microinjected into
oocytes. In some experiments, recovered
round
spermatidswere cultured under co-
culture systemsusing
Sertoli cellsas a feeder cell. Under the co-
culture system, developed
elongating
spermatidscould stimulate and fertilized mature
oocytes. These results indicate that the start of
oocyte activationappearance is between the stage of
round
spermatidand
elongating
spermatidsand the activation ability increases with the advance of spermiogensis. On the other hand,
round
spermatidsisolated from males of ICR strain mouse already have the
oocyte activationability and the fertilizing ability. The result obtained suggests that the expression time of the
oocyte activatingability is difficult between the mouse strain.
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