Analytic and Chemometric Assessments of the Native Probiotic Bacteria and Inulin Effects on Bioremediation of Lead Salts.

BACKGROUND Lead (Pb2+ ) is one of the most toxic heavy metals, which can be found in various quantities in the environment. The five native probiotic bacteria and inulin were used to assess in vitro lead nitrate and lead acetate binding capacities and removal potentials. RESULTS The highest decrease in the media's pH was seen in samples containing a combination of L. paracasei IRBC-M 10784, lead nitrate, and inulin (5.30 ± 0.012). The presence of inulin in the environment accelerated decreases in the pH of all samples with no significance. In all groups, lead nitrate-containing samples included maximum pH decreases. From the highest to the lowest, the ability of lead removal was linked to L. acidophilus PTCC-1932 (88.48%), B. bifidum BIA-7 (85.32%), B. lactis BIA-6 (85.24%), L. rhamnosus IBRC-M 10782 (83.18%) and, L. paracasei IRBC-M 10784 (80.66%). Most species included the highest decrease in lead nitrate. FTIR analysis demonstrated that various functional groups (hydroxyl, carboxylic, carbonyl, amino, and amide binds) on the bacterial cell wall were involved in lead ion binding during incubation. Principal component analysis (PCA) of the FTIR results showed differences in treated groups and control groups. CONCLUSION These results have revealed that the simultaneous use of native probiotics and inulin can be an effective and safe approach for removing various toxic substances, especially Pb. This article is protected by copyright. All rights reserved.