Static stretch affects neural stem cell differentiation in an extracellular matrix-dependent manner.
2015
Neural stem and progenitor cell (NSPC) fate is strongly influenced by
mechanotransductionas modulation of substrate stiffness affects lineage choice. Other types of mechanical stimuli, such as stretch (tensile strain), occur during CNS development and trauma, but their consequences for NSPC differentiation have not been reported. We delivered a 10% static equibiaxial stretch to NSPCs and examined effects on differentiation. We found
static stretchspecifically impacts NSPC differentiation into
oligodendrocytes, but not neurons or astrocytes, and this effect is dependent on particular extracellular matrix (ECM)-integrin linkages. Generation of
oligodendrocytesfrom NSPCs was reduced on
laminin, an outcome likely mediated by the α6
laminin-binding integrin, whereas similar effects were not observed for NSPCs on fibronectin. Our data demonstrate a direct role for tensile strain in dictating the lineage choice of NSPCs and indicate the dependence of this phenomenon on specific substrate materials, which should be taken into account for the design of biomaterials for NSPC transplantation.
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