A pro- and an anti-inflammatory cytokine are synthetised in distinct brain macrophage cells during innate activation
2005
Abstract Brain macrophages are known to exert dual and opposing functions on neuronal survival, which can be either beneficial or detrimental. The rationale of our study is that this duality could arise from an exclusive secretion of either pro- or anti-inflammatory
cytokineby distinct cell subsets,
cytokinesthat could respectively mediate neurotoxic or neurotrophic effects. Innate immune response was induced in macrophage cultures prepared from embryonic-day-16 to postnatal-day-8 mouse brains. By immunofluorescent detection of intracellular
cytokines, we have assessed the occurrence of TNFα or IL10 synthesis at single cell level and observed distinct secretory patterns that include cells producing exclusively TNFα or IL10, cells producing both
cytokinesand non-producer cells. These secretory patterns are differentially regulated by MAP-kinase inhibitors. Altogether, these results demonstrate that synthesis of either a pro- or an anti-inflammatory
cytokinecan segregate distinct brain macrophages and suggests a functional cell-subset-specialisation.
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