Micronomicin/tobramycin binding with DNA: fluorescence studies using of ethidium bromide as a probe and molecular docking analysis
2019
Two aminoglycosides,
micronomicin(MN), and
tobramycin(TB), binding with DNA were studied using various spectroscopic techniques including fluorescence, UV–Vis, FT-IR, and CD
spectroscopycoupled with
relative viscosityand molecular docking. Studies of fluorescence quenching and time-resolved fluorescence
spectroscopyall revealed that MN/TB quenching the fluorescence of DNA–EB belonged to static quenching. The
binding constantsand binding sites were obtained. The values of ΔH, ΔS, and ΔG suggested that
van der Waals forceor hydrogen bond might be the main binding force. FT-IR and CD
spectroscopyrevealed that the binding of MN/TB with DNA had an effect on the secondary structure of DNA. Binding mode of MN/TB with DNA was groove binding which was ascertained by viscosity measurements, CD
spectroscopy, ionic strength, melting temperature (Tm), contrast experiments with single stranded (ssDNA), and double stranded DNA (dsDNA). Molecular docking analysis further confirmed that the groove binding was more...
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