Spatial and temporally expressed proteins during Drosophila oogenesis as revealed by monoclonal antibodies

By use of hybridoma technique, we have prepared 6 monoclonal antibodies. As shown by antibody labeling of whole mount ovary, four of them recognized, respectively, the antigens which were spatial-temporally expressed during oogen-esis. The B 2 antigens appeared very early in the germarium and were expressed mainly by cystocyte and nurse cells.Later, they were all transported and localized in the posterior of oocyte, F 9 antigens followed and were also transported and lo- calized in the posterior of oocyte in the stage 7-8. Then, E 8 antigens appeared and mainly localized on the membrane of oocyte in the stage 9-10. C 3 antigens were expressed much later, at about stage 14.They localized like two caps in the perivitelline fluid at both ends of matured egg. Such specific expression and distribution pattern of all these antigens suggest their possible roles during ooge-nesis.