Whole-Blood miRNA Sequencing Profiling for Vasospasm in Patients With Aneurysmal Subarachnoid Hemorrhage

Background and Purpose— Arterial vasospasmis a well-known delayed complication of aneurysmal subarachnoid hemorrhage (aSAH). However, no validated biomarker exists to help clinicians discriminating patients with aSAH who will develop vasospasm(VSP+) and identifying those who then deserve aggressive preventive therapy. We hypothesized that whole-bloodmiRNAs could be a source of candidate biomarkers for vasospasm. Methods— Using a next-generation sequencing approach, we performed whole-bloodmiRNA profiling between VSP+patients with aSAH and patients who did not develop vasospasm(VSP−) in a prospective cohort of 32 patients. Profiling was performed on the admission day and 3 days before vasospasm. Results— Four hundred forty-two miRNAs were highly expressed in whole bloodof patients with aSAH. Among them, hsa-miR-3177-3p demonstrated significant (P=5.9×10−5; PBonferroni corrected=0.03) lower levels in VSP− compared with VSP+ patients. Looking for whole-bloodmRNA correlates of hsa-miR-3177-3p, we obser...