Effects and Mechanism of the Inhibition of H22 Hepatocarcinoma Cell Growth by Cartilage Polysaccharide

The effect and mechanism of inhibition of H22 Hepatocarcinoma cell growth by cartilage polysaccharides (CA) were studied. Transplanted H22 hepatocarcinoma was used as the model and the mice were randomly divided into 2 group, the control group and CA group. The growth and apoptosis of the tumor cell were investigated by HE Staining, terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL), flow cytometry (FCM) and immunofluorescent staining. Results showed that CA could induce the apoptosis of the H22 cell, inhibit the growth of tumor and prolong the life span time of the examined mice. Besides, increasing the time after taking medicine could enhance the expression of P21 protein in the CA group and inhibit the expression of Cyclin D1. As a new anti-cancer agent, CA had wide application prospects.