Abstract 1981: M2-macrophage derived exosomes mediates tumor progression via GATA3-IL-4-IL-13 axis in ovarian cancer

Tumor microenvironmentis the key factor for tumor progression. Stromal cells which consist of, tumor associated macrophages(TAMs), tumor associated fibroblast as well as cancer stem cells has been implicated in the acquisition of oncogenic phenotype via cell-cell communication. Exosomesare the major players in tumor microenvironmentthat contributes to tumorigenesis. Exosomescarry genetic information from host cells to another cell to reprogram the recipient cell. Exosomesfrom TAMS has emerged as important mediators of tumor growth. Here, we report that GATA3is released abundantly from TAM cells via exosomesand promote oncogenesis. GATA3acts as an oncogenic protein and silencing GATA3by siRNA led to decrease in tumor cell viability. Our data showed that silencing GATA3increased the release of IL-4 and IL-13 from EOC cells, which leads to M2 cell polarizationfrom M0 cells. Our results suggest that GATA3released from macrophage exosomescontributes to tumor growth by affecting intracellular processes as well as tumor microenvironment. P.K. and A. El-A. contributed equally. Citation Format: Pinar Kanlikilicer, Amr El-Arabey, Merve Denizli, Recep Bayraktar, Bulent Ozpolat, Gabriel Lopez-Berestein, S A. Salama, A R. Abd-Allah. M2-macrophage derived exosomesmediates tumor progressionvia GATA3-IL-4-IL-13 axis in ovarian cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 1981.