Construction of high-resolution genetic linkage map in pear pseudo-BC 1 (( Pyrus pyrifolia × P. communis ) × P. pyrifolia ) using GBS-SNPs and SSRs

Genetic linkage map of pear ((Pyrus pyrifolia × P. communis) × P. pyrifolia) was constructed to validate the effectiveness of the two-enzyme approach in GBS library preparation. In addition, allele inheritance was analyzed to investigate the usefulness of the pear pseudo-BC1 in genetic analysis. A total of 905 GBS-SNPs and 69 SSRs were anchored in 17 linkage groups with total genetic distance of 1760.1 cM and average marker distance of 1.88 cM. The genetic linkage map represents 80.5% of pear genome. GBS two-enzyme approach improved marker density and genome coverage compared to the single-enzyme approach. In addition, the inheritance analysis of SSR alleles demonstrated that pseudo-BC could allow genetic dissection of donor parent specific trait segregating in the F2. The high-resolution genetic linkage map of pear pseudo-BC1 will be used for candidate gene identification. Moreover, genetic analysis of donor parent specific traits segregating in the pseudo-BC1 hybrids will promote marker development for molecular breeding in pear.