Phenotypical and morphological analyses of intraepithelial and lamina propria lymphocytes in normal and regenerating gastric mucosa of rats in comparison with those in intestinal mucosa.
2000
While the intestine has abundant
intraepithelial lymphocytes(IELs) including extrathymically differentiated T-cell populations and natural killer (NK) cells, the stomach contains only a few IELs. To elucidate whether the
gastric epitheliumis capable of inducing predominant lymphocyte lodging and subsequent differentiation within, we counted the number of IELS and
lamina proprialymphocytes (LPLs) and calculated the percentage of IELS to total lymphocytes for each alpha-beta (αβ) T cell, gamma-dalta (γδ) T cell, CD4+ cell, CD8+ cell and NK cell in normal and regenerating
gastric mucosaas well as the intestinal mucosa of the rat. In the normal rat
pylorus, a few αβT cells but no γδT cells were found in the
epitheliumand
lamina propria. In regenerating
gastric mucosa, all subsets of LPLs increased in number to a degree comparable to those in intestinal mucosa, whereas every IEL subset, though slightly increased, was much smaller in number than in the intestinal mucosa, consequently giving lower percentages of IELs. Electron microscopic observations revealed that all IELs in regenerating
gastric mucosawere agranular, while 55% of intestinal IELs were large granular lymphocytes positively stained for an NK-cell, αβ-cell or γδT-cell marker. The present results indicate that, unlike the
intestinal epithelium, the
gastric epitheliumdoes not induce the preferential localization of T cells/NK cells and T-cell differentiation into granular lymphocytes in the
epitheliumeven under conditions of prominent LPL infiltration.
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