Yeast Kluyveromyces lactis as host for expression of the bacterial lipase: cloning and adaptation of the new lipase gene from Serratia sp.

Many microbial lipaseshave been successfully expressed in yeasts, but not in industrially attractive Kluyveromyces lactis, which among other benefits can be cultivated on a medium supplemented with whey––cheap and easily available industrial waste. A new bacterial lipasefrom Serratiasp. was isolated and for the first time expressed into the yeast Kluyveromyces lactisby heterologous protein expression system based on a strong promoter of Kluyveromyces marxianus triosephosphate isomerasegene and signal peptideof Kluyveromyces marxianusendopolygalacturonase gene. In addition, the bacterial lipasegene was synthesized de novo by taking into account a codon usage biasoptimal for K. lactis and was expressed into the yeast K. lactis also. Both resulting strains were characterized by high output level of the target proteinsecreted extracellularly. Secreted lipaseswere characterized for activity and stability.