Proteome Profiling of Diabetic Mellitus Patient Urine for Discovery of Biomarkers by Comprehensive MS-Based Proteomics
2018
Diabetic mellitus (DM) is a disease that affects glucose homeostasis and causes complications, such as
diabetic nephropathy(DN). For early diagnosis of DN,
microalbuminuriais currently one of the most frequently used biomarkers. However, more early diagnostic biomarkers are desired in addition to
microalbuminuria. In this study, we performed comprehensive proteomics analysis of urine proteomes of diabetic mellitus patients without
microalbuminuriaand healthy volunteers to compare the protein profiles by mass spectrometry. With high confidence criteria, 942 proteins in healthy volunteer urine and 645 proteins in the DM patient urine were identified with label-free semi-quantitation, respectively. Gene ontology and
pathway analysiswere performed with the proteins, which were up- or down-regulated in the DM patient urine to elucidate significant changes in pathways. The discovery of a useful biomarker for early DN discovery is expected.
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