Rational approach to highly potent and selective apoptosis signal-regulating kinase 1 (ASK1) inhibitors.
2017
Abstract Many diseases are believed to be driven by pathological levels of reactive oxygen species (ROS) and oxidative stress has long been recognized as a driver for
inflammatory disorders. Apoptosis signal-regulating kinase 1 (
ASK1) has been reported to be activated by intracellular ROS and its inhibition leads to a down regulation of p38-and JNK-dependent signaling. Consequently,
ASK1inhibitors may have the potential to treat clinically important inflammatory pathologies including renal, pulmonary and liver diseases. Analysis of the
ASK1ATP-binding site suggested that Gln756, an amino acid that rarely occurs at the GK+2 position, offered opportunities for achieving kinase selectivity for
ASK1which was applied to the design of a parallel
medicinal chemistrylibrary that afforded inhibitors of
ASK1with nanomolar potency and excellent
kinomeselectivity. A focused optimization strategy utilizing structure-based design resulted in the identification of
ASK1inhibitors with low nanomolar potency in a cellular assay, high selectivity when tested against kinase and broad pharmacology screening panels, and attractive physicochemical properties. The compounds we describe are attractive tool compounds to inform the therapeutic potential of
ASK1inhibition.
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