Establishment and characterization of a cisplatin‐resistant cell line (IGSK‐1) from a poorly differentiated gastric adenocarcinoma
2007
We successfully established a spontaneously cisplatin-resistant tumor cell line (designated as IGSK-1) derived from original gastric carcinoma. The patient was a 75-year-old Japanese woman. The histopathological diagnosis was gastric poorly differentiated adenocarcinoma accompanied with metastatic foci in lymph nodes, pT3, N2 M0, stage IIIB. The IGSK-1 cells grew as adhesive and monolayered cultures on the bottom of dishes. The susceptibility of the IGSK-1 cells to anti-cancer drugs was examined using oxygen electrode apparatus (Daikin, Tsukuba, JPN), and the results suggested TXL was effective, and CDDP, CPT-11 and 5-FU were not effective.
Gastrinand
somatostatinsecretions were confirmed by immunohistochemical staining and also
radioimmunoassay. Immunohistochemistry and
radioimmunoassayfor serotonin suggested the IGSK-1 cells might incorporate serotonin from the growth media. Spontaneously cisplatin-resistant gastric carcinoma cell line secreted
gastrinand
somatostatinis very important material for chemotherapy.
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