Supporting Material, Supporting Figures S1-S4, Supporting Tables S1-S6 from Polyclonal symbiont populations in hydrothermal vent tubeworms and the environment
2019
Horizontally transmitted
symbiosesusually house multiple and variable symbiont genotypes that are acquired from a much more diverse environmental pool via partner choice mechanisms. However, in the deep-sea
hydrothermal venttubeworm Riftia
pachyptila(Vestimentifera,
Siboglinidae), it has been suggested that the
CandidatusEndoriftia persephone symbiont is monoclonal. Here, we show with high-coverage
metagenomicsthat adult R.
pachyptilahouse a polyclonal symbiont population consisting of one dominant and several low-frequency variants. This dominance of one genotype is confirmed by multilocus gene sequencing of amplified
housekeeping genesin a broad range of host individuals where three out of four loci (atpA, uvrD and recA) revealed no genomic differences, while one locus (gyrB) was more diverse in adults than in juveniles. We also analysed a
metagenomeof free-living Endoriftia and found that the free-living population showed greater sequence variability than the host-associated population. Most juveniles and adults shared a specific dominant genotype, while other genotypes can dominate in few individuals. We suggest that although generally permissive, partner choice is selective enough to restrict uptake of some genotypes present in the environment.
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