Array comparative genomic hybridization analysis revealed four genomic prognostic biomarkers for primary gastric cancers

Abstract Unlike the case with some other solid tumors, whole genome array screening has not revealed prognosticgenetic aberrations in primary gastric cancer. Comparative genomic hybridization(CGH) using bacterial artificial chromosome(BAC) arrays for 56 primary gastric cancers resulted in identification of four prognosticloci in this study: 6q21 (harboring FOXO3A ; previously FKHRL1 ), 9q32 ( UGCG ), 17q21.1∼q21.2 ( CASC3 ), and 17q21.32 ( HOXB3 through HOXB9 ). If any one of these four loci was deleted, the prognosis of the patient was significantly worse ( P = 0.0019). This was true even for advanced tumors (stage IIIA, IIB, or IV, n = 39) ( P = 0.0113), whereas only 1 of the 17 patients with less advanced tumors (stage IA, IB, or II; n = 17) died of disease after surgery. Multivariate analysis according to the status of four BACs or pathological stage based on the Japanese Classification of Gastric Carcinoma (stages IA, IB, and II vs. stages IIIA, IIIB, and IV) demonstrated that the BAC clone status was also an independent prognosticfactor ( P = 0.006). These findings may help predict which patients with malignant potential need more intensive therapy, or may point to new therapeutic approaches especially for advanced tumors. The parameter here termed the integrated genomic prognosticbiomarker may therefore be of clinical utility as a prognosticbiomarker.