Processing Human Thymic Tissue for Single Cell RNA-Seq

Summary Single cell RNA sequencing of human thymic cells is dependent on isolation of highly pure and viable cell populations. This protocol describes the isolation of CD34+ progenitor and more differentiated CD34– fractions from post-natal thymic tissue to study thymopoiesis. CD34+ cells represent For complete details on the use and execution of this protocol, please refer to Le et al. (2020) .