High-resolution analysis of the human T-cell receptor repertoire
2015
Unbiased dissection of
T-cell receptor(TCR)
repertoirediversity at the nucleotide level could provide important insights into human immunity. Here we show that TCR ligation-anchored-magnetically captured PCR (TCR-LA-MC PCR) identifies TCR α- and β-chain diversity without sequence-associated or quantitative restrictions in healthy and diseased conditions. TCR-LA-MC PCR identifies convergent recombination events, classifies different stages of
cutaneous T-cell lymphomain vivo and demonstrates TCR reactivation after in vitro cytomegalovirus stimulation. TCR-LA-MC PCR allows ultra-deep
data accessto both physiological TCR diversity and mechanisms influencing clonality in all clinical settings with restricted or distorted TCR
repertoires.
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