Differentiation Kinetics of Blood Monocytes and Dendritic Cells in Macaques: Insights to Understanding Human Myeloid Cell Development

Monocyteand dendritic cell (DC) development was evaluated using in vivo BrdU pulse-chase analyses in rhesus macaques, and phenotype analyses of these cells in blood also were assessed by immunostaining and flow cytometry for comparisons among rhesus, cynomolgus, and pigtail macaques, as well as African green monkeysand humans. The nonhuman primate species and humans have three subsets of monocytes, CD14+ CD16− , CD14+ CD16+ , and CD14− CD16+ cells, which correspond to classical, intermediate, and nonclassical monocytes, respectively. In addition, there exist presently two subsets of DC, BDCA-1 + myeloidDC and CD123 + plasmacytoid DC, that were first confirmed in rhesus macaqueblood. Following BrdU inoculation, labeled cells first appeared in CD14+ CD16− monocytes, then in CD14+ CD16+ cells, and finally in CD14− CD16+ cells, thus defining different stages of monocytematuration. A fraction of the classical CD14+ CD16− monocytesgradually expressed CD16+ to become CD16+ CD14+ cells and subsequently matured into the nonclassical CD14− CD16+ cell subset. The differentiation kinetics of BDCA-1 + myeloidDC and CD123 + plasmacytoid DC were distinct from the monocytesubsets, indicating differences in their myeloidcell origins. Results from studies utilizing nonhuman primates provide valuable information about the turnover, kinetics, and maturation of the different subsets of monocytesand DC using approaches that cannot readily be performed in humans and support further analyses to continue examining the unique myeloidcell origins that may be applied to address disease pathogenesis mechanisms and intervention strategies in humans.