Investigation of inter- and intraspecies variation through genome sequencing of Aspergillus section Nigri
2018
Aspergillussection Nigri comprises filamentous fungi relevant to
biomedicine,
bioenergy, health, and biotechnology. To learn more about what genetically sets these species apart, as well as about potential applications in biotechnology and
biomedicine, we sequenced 23 genomes de novo, forming a full genome
compendiumfor the section (26 species), as well as 6
Aspergillusniger isolates. This allowed us to quantify both inter- and intraspecies genomic variation. We further predicted 17,903 carbohydrate-active enzymes and 2,717 secondary metabolite
gene clusters, which we condensed into 455 distinct families corresponding to compound classes, 49% of which are only found in single species. We performed metabolomics and genetic engineering to correlate genotypes to phenotypes, as demonstrated for the metabolite aurasperone, and by heterologous transfer of citrate production to
Aspergillus nidulans. Experimental and computational analyses showed that both
secondary metabolismand regulation are key factors that are significant in the delineation of
Aspergillusspecies.
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