Pancreatic islet characterisation by the hyperspectral Assessment of autofluorescence: a non-invasive, label-free measure of viability

Type 1 diabetes occurs when insulin secreting beta cells in pancreatic islets are destroyed leading to elevated glucose and ill health. Islet transplantation is an effective therapy, but islets are often damaged by the isolation process resulting in numerous, repeated transplants to achieve insulin independence. We have applied hyperspectral microscopy to damaged islets and have shown through the assessment of native cell autofluorescence we can detect heterogenous forms of damage (elevated ROS, inflammatory signalling and warm ischemia) in mouse islets. This approach has great potential to be translated clinically to minimise the burden of suboptimal islet transplantations.