Evaluation of OMNIgene®•SPUTUM reagent for mycobacterial culture
2018
: National Mycobacterium Reference Laboratory, Borstel, Germany. : To evaluate the effectiveness of OMNIgene®•SPUTUM (OM-S)
reagentin comparison with a method using
N-acetyl-L-cysteine-sodium hydroxide (NALC-NaOH) with regard to mycobacterial recovery and contamination of broth and solid cultures. : Sputum samples from patients with tuberculosis and other respiratory diseases underwent decontamination with NALC-NaOH-based (MycoDDR™) or OM-S
reagent. The decontamination procedure was assigned by block randomisation. Samples were inoculated on Lowenstein-Jensen, Stonebrink and MGIT™ (Mycobacterial Growth Indicator Tubes). Mycobacterial recovery from samples spiked with Mycobacterium tuberculosis following decontamination was determined. : Eighty-five samples were randomised to NALC-NaOH and 84 to OM-S
reagent. Mycobacterial recovery was significantly lower for samples processed with OM-S
reagentcompared with the NALC-NaOH method across all
media types. Culture contamination was lower with NALC-NaOH
reagenton solid media (9.4-12.9% vs. 28.6-29.8%). Growth was not observed in MGIT among samples spiked with 10 600-16 800 colony-forming units of M. tuberculosis following decontamination with OM-S
reagent. : Low mycobacterial recovery, especially in MGIT, observed in the present study suggests that OM-S
reagentmight not be compatible with the MGIT system. More extensive field evaluations of the OM-S
reagentare warranted to demonstrate a significant benefit over currently used methods.
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