Identification of candidate flavonoid pathway genes using transcriptome correlation network analysis in ripe strawberry (Fragaria × ananassa) fruits
2015
New modulators of the strawberry
flavonoidpathway were identified through correlation network analysis. The transcriptomes of red, ripe fruit from two parental lines and 14 of their progeny were compared, and uncharacterized transcripts matching the expression patterns of known
flavonoid-pathway genes were identified. Fifteen transcripts corresponded with putative transcription factors, and several of these were examined experimentally using transient expression in developing fruits. The results suggest that two of the newly-identified regulators likely contribute to discrete nodes of the
flavonoidpathway. One increases only
LEUCOANTHOCYANIDIN REDUCTASE(LAR) and
FLAVONOL3’-HYDROXYLASE (F3’H) transcript accumulation upon overexpression. Another affects LAR and
FLAVONOL SYNTHASE(FLS) after overexpression. The third putative transcription factor appears to be a universal regulator of
flavonoid-pathway genes, as many pathway transcripts decrease in abundance when this gene is silenced. This report demonstrates that such systems-level approaches may be especially powerful when connected to an effective transient expression system, helping to provide rapid and strong evidence of gene function in key fruit-ripening processes.
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