Multi-Granulin Domain Peptides Bind to Pro-Cathepsin D and Stimulate Its Enzymatic Activity More Effectively Than Progranulin in Vitro
2019
Progranulin (PGRN) is an evolutionarily conserved glycoprotein associated with several disease states, including neurodegeneration, cancer and autoimmune disorders. This protein has recently been implicated in the regulation of lysosome function, whereby PGRN may bind to and promote the maturation and activity of the aspartyl protease
cathepsin D(proCTSD – inactive precursor, matCTSD – mature, enzymatically active form). As the full-length PGRN protein can be cleaved into smaller peptides, called
granulins, we assessed the function of these
granulinpeptides in binding to proCTSD and stimulating matCTSD enzyme activity in vitro. Here, we report that full-length PGRN and multi-
granulindomain peptides bound to proCTSD with low- to sub-micromolar binding affinities. This binding promoted proCTSD destabilization, which was greater in magnitude for multi-
granulindomain peptides than for full-length PGRN. Such destabilization correlated with enhanced matCTSD activity at acidic pH. The presence and function o...
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