In situ measurement of absolute concentrations by Normalized Raman Imaging

We introduce Normalized Raman Imaging (NoRI), a Stimulated Raman Scattering (SRS) microscopy method that computationally removes the effect of tissue light scattering. NoRI provides high resolution measurements of the absolute concentration of total protein, total lipid and water from live or fixed thick tissue samples with single cell resolution, and can also be applied to other Raman bands. NoRI enables study of the protein, lipid, and water concentration variation associated with development and diseases.