Encapsulation and storage of therapeutic fibrin-homing peptides using conducting polymer nanoparticles for programmed release by electrical stimulation
2020
Cys-Arg-Glu-Lys-Ala
(CREKA) is an important fibrin-homing pentapeptide
that has been extensively demonstrated for diagnoses and therapies
(e.g., image diagnosis of tumors and to inhibit tumor cell migration
and invasion). Although CREKA-loaded nanoparticles (NPs) have received
major interest as efficient biomedical systems for
cancer diagnosis and treatment, almost no control on the peptide
release has been achieved yet. Herein, we report the development
of conductive polymer NPs as therapeutic CREKA carriers for controlled
dose administration through electric stimuli. Furthermore, the study
was extended to CR(NMe)EKA, a previously engineered
CREKA analogue in which Glu was replaced by N-methyl-Glu
for improvement of the peptide resistance against proteolysis, which
is one of the major weaknesses of therapeutic peptide delivery, and
for enhancement of the tumor homing capacity by overstabilizing the
bioactive conformation. Particularly, the present work is focused
on understanding the interactions between the newly designed nanoengineered
materials and biological fluids and the achievement of a modulated
peptide release by fine-tuning the electrical stimuli. Two different types of stimuli were compared, chronoamperometry
versus cyclic voltammetry, the latter being more effective.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
61
References
14
Citations
NaN
KQI