Patterns of IgE sensitization in house dust mite-allergic patients: implications for allergen immunotherapy
2016
BACKGROUND: Understanding patterns of IgE sensitization in Dermatophagoides-allergic patients living in various geographical areas is necessary to design a product suitable for worldwide
allergen immunotherapy(AIT). METHODS: Using a HIFI Allergy customized microarray assay, IgEs specific for 12 purified
allergensfrom Dermatophagoides pteronyssinus or D. farinae were assessed in sera from 1302
house dust mite(HDM)-allergic patients living in various areas. Comprehensive mass spectrometric (MS) analyses were conducted to characterize HDM extracts, as well as purified bodies and
feces. RESULTS: Patterns of IgE reactivity to HDM
allergensare comparable in all cohorts of patients analyzed, encompassing adults and 5- to 17-year-old children, as well as American, Canadian, European, and Japanese patients. Overall, >70% and >80% of HDM-allergic patients are sensitized to group 1 and group 2
allergens, respectively, from D. pteronyssinus and/or D. farinae species. Furthermore, 20-47% of patients also have IgEs to
allergensfrom groups 4, 5, 7, 13, 15, 21, and 23. All patients have IgEs to
allergenspresent in mite bodies and
feces. MS-based analyses confirmed the presence of mite
allergensrecorded by IUIS in D. pteronyssinus and D. farinae extracts, with groups 2, 8, 10, 11, 14, and 20 prominent in bodies and groups 1, 6, 18, and 23 well represented in
feces. CONCLUSIONS: Mite-specific AIT should rely upon a mixture of D. pteronyssinus and D. farinae extracts, manufactured from both
fecesand bodies. Such a combination is appropriate to treat children and adult Dermatophagoides-allergic patients from Asia, Europe, and North America.
Keywords:
-
Correction
-
Source
-
Cite
-
Save
41
References
60
Citations
NaN
KQI