Development of Automated Nuclear Transplantation System
2001
In nuclear transplantation, especially in
enucleation,
rotatingthe
oocyteto a certain position has been an important work. Therefore, we developed an
oocyte
rotation systemby
using
electrostatic
force, which is known to be excellent in handling minute objects. This system has become the basic technology for the automated nuclear transplantation system. Mouse oocyles were
rotatedat a speed of about 60 deg/s by
electrostaticphenomena (i.e.
electrorotationand
dielectrophoresis), and the direction of
rotationcould be reversed. In order to confirm the
electrostaticeffect on the fertilization and developmental ability of
oocytes, we performed IVF and nuclear transplantation with
rotated
oocytes. The result was that the fertilization and developmental ability of the
rotated
oocyteswas the same as that of the control, proving that
rotationby
electrostaticforce does not influence the fertilization and development ability of
oocytes.
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