Enabling 1536-Well High-Throughput Cell-Based Screening through the Application of Novel Centrifugal Plate Washing:
2017
Cell-based
assayshave long been important within hit discovery paradigms; however, improving the disease relevance of the
assaysystem can positively affect the translation of small-molecule drug discovery, especially if adopted in the initial hit identification
assay. Consequently, there is an increasing need for disease-relevant
assaysystems capable of running at large scale, including the use of
induced pluripotent stem cellsand donor-derived
primary cells. Major hurdles to adopting these
assaysfor
high-throughput screeningare the cost, availability of cells, and complex protocols. Miniaturization of such
assaysto 1536-well format is an approach that can reduce costs and increase throughput. Adaptation of these
complex cell
assaysto 1536-well format brings major challenges in liquid handling for high-content
assaysrequiring washing steps and coating of plates. In addition, problematic
edge effectsand reduced
assayquality are frequently encountered. In this study, we describe the novel applica...
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