Clinical and molecular characterisation of hereditary and sporadic metastatic colorectal cancers harbouring microsatellite instability/DNA mismatch repair deficiency
2017
Abstract Background Patients treated with chemotherapy for microsatellite unstable (MSI) and/or mismatch repair deficient (dMMR) cancer metastatic colorectal cancer (mCRC) exhibit poor prognosis. We aimed to evaluate the relevance of distinguishing sporadic from
Lynch syndrome(LS)-like mCRCs. Patients and methods MSI/dMMR mCRC patients were retrospectively identified in six French hospitals. Tumour samples were screened for MSI, dMMR, RAS / RAF mutations and
MLH1methylation. Sporadic cases were molecularly defined as those displaying
MLH1/
PMS2loss of expression with BRAF
V600Eand/or
MLH1hypermethylation and no MMR
germline mutation. Results Among 129 MSI/dMMR mCRC patients, 81 (63%) were LS-like and 48 (37%) had sporadic tumours; 22% of
MLH1/
PMS2-negative mCRCs would have been misclassified using an algorithm based on local medical records (age, Amsterdam II criteria, BRAF and MMR statuses when locally tested), compared to a systematical assessment of MMR, BRAF and
MLH1methylation statuses. In univariate analysis, parameters associated with better overall survival were age ( P P = 0.001) and LS-like mCRC ( P = 0.01), but not BRAF
V600E. In multivariate analysis, age (hazard ratio (HR) = 3.19, P = 0.01) and metastatic resection (HR = 4.2, P = 0.001) were associated with overall survival, but not LS. LS-like patients were associated with more frequent liver involvement, metastatic resection and better disease-free survival after
metastasectomy(HR = 0.28, P = 0.01). Median progression-free survival of first-line chemotherapy was similar between the two groups (4.2 and 4.2 months; P = 0.44). Conclusions LS-like and sporadic MSI/dMMR mCRCs display distinct natural histories. MMR, BRAF mutation and
MLH1methylation testing should be mandatory to differentiate LS-like and sporadic MSI/dMMR mCRC, to determine in particular whether
immune checkpointinhibitors efficacy differs in these two populations.
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