Drosophila NAT1, a homolog of the vertebrate translational regulator NAT1/DAP5/p97, is required for embryonic germband extension and metamorphosis.
2007
Translational regulationhas been to shown to play major roles in the patterning of the early Drosophila embryo. The
eIF4Gfamily member NAT1/p97/DAP5 has been identified as a novel translational repressor. To genetically dissect the in vivo function of this unconventional
eIF4G-related
translational regulator, Drosophila NAT1 (dNAT1) mutants were isolated using a
reverse-geneticsapproach. Four transposon insertion mutants and a deletion mutant affecting the dNAT1 locus were analyzed. Genetic complementation tests and germline rescue using a 12 kb dNAT1 genomic DNA fragment revealed these to be loss-of-function mutants. One
P-elementinsertion line, dNAT1GS1., shows severe embryonic lethality and abnormal germband extension. Abnormalities at metamorphosis were also found, including defective head eversion and salivary gland degeneration in the hypomorphic allele dNATex1. A phenotypic analysis of dNAT1 mutants suggests that dNAT protein plays a specific rather than general role in
translational regulation.
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