Differentially expressed plasma proteins of β-thalassemia/hemoglobin E patients in response to curcuminoids/vitamin E antioxidant cocktails

ABSTRACTObjective: Iron overload and oxidative stress are the major causes of serious complications and mortality in thalassemic patients. Our previous work supports the synergistic effects of antioxidant cocktails ( curcuminoidsor vitamin E, N- acetylcysteine, and deferiprone) in treatment of β- thalassemia/Hb E patients. This further 2-DE-based proteomic study aimed to identify the plasma proteins that expressed differentially in response to antioxidant cocktails.Methods: Frozen plasma samples of ten normal subjects and ten β- thalassemia/Hb E patientsat three-time points (baseline, month 6, and month 12) were reduced the dynamic range of proteome using ProteoMiner kit and separated proteins by two-dimensional gel electrophoresis. Differentially expressed proteins were identified using tandem mass spectrometry. Several plasma proteins were validated by ELISA and Western blot analysis.Results: Thirteen and 11 proteins were identified with altered expression levels in the curcuminoids- and vitamin E cocktai...