Simultaneous analysis of Paracetamol and Tramadol - Analytical method development and validation

In the present study the development and validation of analytical method was carried out for simultaneous analysis of paracetamol and tramadol using first derivative method. The λ max was determined for paracetamol and tramadol and standard curves were plotted. The stability of the stock solutions with due course of time was also determined and compared using t-Test. Further the simultaneous analysis at 1:1, 8:1 and 1:8 ratios revealed the need of a higher derivative method for the simultaneous estimation of paracetamol and tramadol in various dosage forms.