The RNA-editing enzyme ADAR promotes lung adenocarcinoma migration and invasion by stabilizing FAK
2017
Large-scale, genome-wide studies report that
RNA binding proteinsare altered in cancers, but it is unclear how these proteins control tumor progression. We found that the
RNA-editingprotein
ADAR(
adenosine deaminaseacting on double-stranded RNA) acted as a facilitator of lung adenocarcinoma (LUAD) progression through its ability to stabilize transcripts encoding
focal adhesionkinase (FAK). In samples from 802 stage I LUAD patients, increased abundance of
ADARat both the mRNA and protein level correlated with tumor recurrence. Knocking down
ADARin LUAD cells suppressed their mesenchymal properties, migration, and invasion in
culture.
Analysisof gene expression patterns in LUAD cells identified
ADAR-associated enrichment of a subset of genes involved in cell migration pathways; among these, FAK is the most notable gene whose expression was increased in the presence of
ADAR. Molecular analyses revealed that
ADARposttranscriptionally increased FAK protein abundance by binding to the FAK transcript and editing a specific intronic site that resulted in the increased stabilization of FAK mRNA. Pharmacological inhibition of FAK blocked
ADAR-induced invasiveness of LUAD cells, suggesting a potential therapeutic application for LUAD that has a high abundance of
ADAR.
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