Characterizing Crude Oil Toxicity to Early-Life Stage Fish Based On a Complex Mixture: Are We Making Unsupported Assumptions?

Numerous studies of the water-soluble fraction (WSF) from crude oil have concluded that polycyclic aromatic hydrocarbons (PAHs) are the primary causative agents for early life stage (ELS) fish toxicity. Noteworthy is the lack of studies demonstrating that the sum of PAHs are capable of causing toxic effects in ELS fish at the low levels claimed (0.1–5 μg/L) without being part of a complex crude oil mixture. Crude oil and the WSF are composed of thousands of other compounds that co-occur and likely contribute to crude oil toxicity. Based on the available data, it appears that the syndrome of effects (lower heart rate, edemas, and morphological abnormalities) for ELS fish exposed to the aqueous fraction of a crude oil mixture is commonly observed in studies exposing fish embryos to high concentrations of a variety of compounds and may be a nonspecific response. We conclude that the available data support the hypothesis that this syndrome of effects is likely the result of baseline toxicity (not receptor bas...