REVERSE PHASE HPLC METHOD FOR DETERMINATION OF LACIDIPINE IN PHARMACEUTICAL PREPARATIONS Research Article

A simple and reliable high-performance liquid chromatography (HPLC) method was developed and validated for Lacidipine in pharmaceutical preparations. The method was developed on Thermo Hypersil RP C-18 column (100 mm x 4.6 mm, 3.5 µm) using a mobile phase of Acetonitrile: water (65 : 35, v/v). The effluent was monitored by PDA detector at 239 nm. The total run time was 7 min with a flow rate of 1.0 ml/min. Calibration curve was linear over the concentration range of 10 – 50 µg/ml. For Intra–day and inter–day precision % RSD values were found to be 0.481% and 0.487% respectively. Recovery of Lacidipine was found to be in the range of 99.26-100.97%. The limits of detection (LOD) and quantification (LOQ) were 0.03 and 0.1 µg/ml, respectively. The developed RP-HPLC method was successfully applied for the quantitative determination of lacidipine in pharmaceutical dosage forms.