Mycoplasma Infection Alters Cancer Stem Cell Properties in Vitro
2016
Cancer cell lines can be useful to model
cancer stem cells. Infection with
Mycoplasmaspecies is an insidious problem in mammalian cell culture. While investigating stem-like properties in early passage melanoma cell lines, we noted poorly reproducible results from an aliquot of a cell line that was later found to be infected with
Mycoplasma hyorhinis. Deliberate infection of other early passage melanoma cell lines aliquots induced variable and unpredictable effects on expression of putative
cancer stem cellmarkers, clonogenicity, proliferation and global gene expression. Cell lines established in stem cell media (SCM) were equally susceptible.
Mycoplasmastatus is rarely reported in
publications usingcultured cells to study the
cancer stem cellhypothesis. Our work highlights the importance of surveillance for
Mycoplasmainfection while using any cultured cells to interrogate tumor heterogeneity.
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