Can Inhibin B Reflect Ovarian Reserve of Healthy Reproductive Age Women Effectively

Objective: The reference range and potential value of inhibin B is still unclear and controversial. The aims of this study were to define the variation trend of inhibin B in healthy women with age and explore its value in reflection of ovarian reserve. Methods: A total of 2524 healthy reproductive age women from eight medical institutes nationwide were recruited. The variation tendency of inhibin B with age was primarily established in the first group of 948 women and validated in another 605. Then, we evaluated the relationship between inhibin B and classic ovarian reserve and function markers. The potency of inhibin B in predicting poor ovarian response was also estimated and compared with FSH. Results: The nomogram showed that inhibin B rapidly decreased after the age of 40. Inhibin B was positively correlated with AMH (R = 0.57, P < 0.001), AFC (R = 0.34, P < 0.001) and testosterone (R = 0.10, P = 0.002), and negatively correlated with FSH (R = -0.35, P < 0.001), LH (R = -0.13, P < 0.001) and FSH/LH (R=-0.18, P < 0.001), while no correlation was found with PRL. Unexpectedly, inhibin B had a slightly higher value than FSH for predicting poor ovarian response (AUC 0.74 vs 0.71). Conclusions: For healthy reproductive age women, the decline of inhibin B can reflect decreased ovarian reserve effectively, having a good consistency with AMH and AFC. More importantly, inhibin B had an advantage in predicting poor ovarian response compared with FSH. These results will be helpful to the clinical application of inhibin B in the evaluation of female ovarian reserve and the assessment of their reproductive capacity. Trial registration：http://clinicaltrials.gov; NCT02294500.