Phytase activity from Lactobacillus spp. in calcium-fortified soymilk.
2010
The presence of phytate in calcium-fortified soymilk may interfere with mineral absorption. Certain lactic
acid bacteria (LAB) produce the enzyme phytase that degrades phytates and therefore may potentially improve mineral
bioavailability and absorption. This study investigates the phytase activity and phytate degradation potential of 7 strains
of LAB including: Lactobacillus acidophilus ATCC4962, ATCC33200, ATCC4356, ATCC4161, L. casei ASCC290, L.
plantarum ASCC276, and L. fermentum VRI-003. Activity of these bacteria was examined both in screening media and
in calcium-fortified soymilk supplemented with potassium phytate. Most strains produced phytase under both conditions
with L. acidophilus ATCC4161 showing the highest activity. Phytase activity in fortified soymilk fermented with L.
acidophilus ATCC4962 and L. acidophilus ATCC4161 increased by 85% and 91%, respectively, between 12 h and 24 h of
fermentation. All strains expressed peak phytase activity at approximately pH 5. However, no phytate degradation could
be observed.
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